Encoded by the CASP3 gene, caspase-3 is a member of the cysteine-aspartic acid protease (caspase) family. Anti-Mouse Secondary Antibodies; Anti-Rat Secondary Antibodies; Anti-Human Secondary Antibodies; Anti-Goat Secondary Antibodies; Anti-Guinea Pig Secondary Antibodies; PDF | Background: Kawasaki disease (KD) is a kind of vasculitis with unidentified etiology. Franais +1 877 302 8632; Contact . Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C. Immunogen. Lyophilized from a 0.2 m filtered solution in PBS with Trehalose. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) Research with confidence - consistent and reproducible results with every batch. The tissue section was then incubated with 1g/ml rabbit anti-Caspase3 Antibody (PB9188) overnight at 4C. Human, Mouse, Rat Recommended Dilutions WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 Contact www.abclonal.com . Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Home . Cell Death Differ. *Small pack size (SP) is supplied either lyophilized or as a 0.2 m filtered solution in PBS. Order anti-Caspase 4 anticorps ABIN7186100. Caspase3 in Jurkat Human Cell Line. (B) Bax, Bcl-2, and Bad. . Predicted band 30 kD. Caspase-2 [Precursor], EC 3.4.22.55, CASP-2, ICH-1 protease, NEDD2 protein, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Caspase-2 subunit p18, Caspase-2 subunit . Anti-Mouse Secondary Antibodies; Anti-Rat Secondary Antibodies; Anti-Human Secondary Antibodies; Anti-Goat Secondary Antibodies; The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. Sequential activation of caspases plays a central . Validated for Western Blotting. Human/Mouse Cleaved Caspase-3 (Asp175) Antibody Summary Species Reactivity Human, Mouse Specificity Detects human and mouse Caspase-3 cleaved at Asp175. . Western blot - Caspase-3 Mouse mAb (A17900) Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (A17900) at 1:1000 dilution. The mouse genotype used for the majority of our studies is B6.Cg-Tg . (C) Mean villus length at indicated times after pIC. Order anti-Caspase 1 anticorps ABIN7146925. Cells were stained (red) and counterstained (green). These antibodies have been verified by Cell treatment, Knockout and Knockdown to confirm specificity to Caspase 3. (House mouse) Additional product information. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted to the wells. Menu Sign . Caspase 9 Antibody detects endogenous levels of total Caspase 9. Formulation. Email: us@affbiotech.com . (1) Publication (s) Target Human CASP3 / Caspase 3 Synonyms Lapin Caspase 4 Polyclonal anticorps Cleaved-Gln81 pour ELISA, IHC, WB. Avoid repeated freeze-thaw cycles. The tissue section was blocked with 10% goat serum. Upon receipt, store it immediately at the temperature recommended below. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Storage & Handling Store at -20C for one year from date of receipt. Order anti-Caspase 3 antibody ABIN3188045. Anti-Caspase 3 Antibody is tested for use in WB, EM, Flow, ICC/IF, IHC-Fr, and IHC-P. Expect a band approximately 31.5 kDa on specific lysates. [PMC free article] [Google Scholar] Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation. Franais +1 877 302 8632; Contact; Login . SUMMARY We have previously shown an increased susceptibility of T cell subsets to anti-Fas-induced apoptosis in human ageing [1]. Get better batch-to-batch reproducibility with a recombinant antibody. Caspase3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 g/mL Human/Mouse Active Caspase3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Request PDF | On Oct 17, 2022, Vahideh Mirzaei and others published -Hydroxybutyrate Diminishes the Apoptotic Cell Death and Demyelination via Altering Bax, Caspase-3, and Bcl2 Levels in the . H&E stained sections of proximal WT mouse small intestine 3 h after (A) PBS or (B) pIC 30 g/g i.p. Caspase-3, as the name implies, is a member of the cysteine-aspartic acid protease (caspase) protein family.Caspase-3 is an effector caspase that is activated by both the intrinsic and extrinsic apoptosis pathways. Boster Bio Anti-Caspase 3/CASP3 Antibody catalog # PA1849. Preparation of anti-mouse VRK1 antibody. E. coli-derived recombinant mouse Caspase-11/Caspase-4 Pro81-Glu266 Accession # P70343 . English +1 877 302 8632; Contact; Login Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) . . Species Reactivity. 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. News; Identification of ATM Protein Kinase Phosphorylation . Synonyms Caspase-7; CASP-7; ICE-like apoptotic protease 3; ICE-LAP3; Apoptotic protease Mch-3; CMH-1; CASP7; MCH3. Mouse Caspase-11/Caspase-4 Antibody Summary. Species Reactivity Hamster, Human, Mouse, Pig, Rat Published Species Human Host / Isotype Mouse / IgG1 Class Monoclonal Type Antibody Clone 31A1067 Conjugate Unconjugated Immunogen Recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria). These antibodies target Caspase 3 in Human, Mouse, Rat, Non-human primate and Goat samples. (D) Full PARP and Cleaved-PARP. Mouse . Mouse anti Human caspase-3 antibody, clone CD3/3D6 recognizes caspase-3, also known as CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1 and protein Yama (protein of death). Cited in 1 publication. Choose the anti-Caspase-3 antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Cleaved-Caspase 3 (Asp175), p17 Antibody. Validated for IHC and WB. The results also show that the rabbit anti- active caspase-3 antibody (clone C92-605) immunoprecipitated only the active form of caspase-3 (lanes 8-11) as compared to the mouse anti-human caspase-3 antibody (Cat. Western blot was performed from the immunoprecipitate using Caspase-1 antibody (A0964) at a dilition of 1:1000. After reconstitution, at 4C for one month. Mouse VRK1 antisera were generated in rabbit using recombinant mouse . we measured GSDMD mitochondrial association by co-staining isolated mitochondria with MitoTracker and anti-GSDMD antibodies after AIM2 activation. Caspase-3 Mouse mAb images. Storage This antibody can be stored at 2-8 for one month without detectable loss of activity. It is reactive with human and mouse. This antibody reacts with Human, Mouse, Rat. The cleaved subunits then form an active heterotetramer by hydrophobic interactions and trigger subsequent caspase cascades to induce the apoptotic phenotype. The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila. To best visualize the immune cell activation to cerebral injury, we chose the following antibodies: CD68/ED1 for macrophages (and activated microglia) (14, 57); CD34 for proliferating microglia and CD43/W3/13 for neutrophils; Activated caspase 3 antibody for cell apoptosis ; and NeuN (Neuronal Nuclei/Fox3) for neurons. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp374) (18C8) Rabbit Monoclonal Antibody (CST #9496) is ready to ship. It can also be aliquotted and stored frozen at -20C for six months. A rabbit polyclonal antibody, raised against Caspase-7, supplied by Assay Biotechnology. Tested in WB applications. . Scale bar 200 m. Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Anti-Caspase-3 Mouse Monoclonal Antibody (CAB17900) SKU: CAB17900 UPC: Product type: Antibody Reactivity: Human Reactivity: Mouse Host Species: Mouse Isotype: IgG Research Area: Cell Death. 2009; 17:534-539. (A) p53 and p-p53. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37C. Current Stock: Quantity: Decrease Quantity: Increase Quantity: Frequently bought together: . This antibody is shipped as liquid solution at ambient temperature. Success from the first experiment - confirmed . Caspase3 was detected in paraffin-embedded section of mouse intestine tissue. Anti-Caspase-3 Rabbit Monoclonal Manufactured by Gentaur. This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody. L1 mouse brain lysates L2 mouse liver lysates probed with Anti pro-caspase-3 Polyclonal Antibody, Unconjugated (bs-2593R) at 1:200 overnight at 4C. Mouse Monoclonal to Active Caspase-3. Caspase 3 antibody LS-B2125 is an unconjugated mouse monoclonal antibody to Caspase 3 (CASP3) from human. Mouse Monoclonal Caspase 3 antibody for IHC, WB. Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Gentaur is the biggest antibody manufacturer worldwide. Comparison between all groups was evaluated through One-way ANOVA. : +1 877 302 8632 : +1 888 205 9894 (Toll-free) : orders@antibodies-online.cn Results are mean SEM value from three separate experiments. Our Caspase-3 antibodies can be used in a variety of model species including Human, Mouse, Rat, Bovine, Canine, Gerbil, Fish, Porcine, Rabbit, and Sheep. Form Liquid Concentration 1mg/mL Purification Protein G Storage . Source Monoclonal Rabbit IgG Clone # 269518 Purification Product Details Target Information This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 1 g/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003 ). Caspase-8 and -10 can also be involved . The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA Fragmentation Factor (DFF). Western blot analysis of extracts of various cell lines, using Caspase-1 antibody (A21085) at 1:1000 dilution. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For more information see here. Lapin Caspase 1 Polyclonal anticorps AA 120-269 pour ELISA, IHC. In this study, we have examined the role of downstream mediators, including caspases, in Fas-mediated apoptosis in Figure 9 The representative photographs and grayscale analysis of proteins in testis from mice treated for 30 days involved in MAPK signaling pathway. Following another washing, 100 L of 1:200 diluted . Our Caspase 3 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit and Mouse. 610322) which immunoprecipitated both the pro and active forms of caspase-3 (lanes 12 and 13). Results represent three different experiments. AF6348 at 1/100 staining Mouse heart tissue by IHC-P. . After 24 h, cell viability was measured by MTT assay (D), and whole-cell extracts were analyzed by immunoblotting with antibodies to cleaved caspase 3 or GAPDH (E). Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively. Gentaur Human Antibodies; Gentaur Monoclonal Antibodies; Gentaur Mouse Antibodies; Gentaur Polyclonal Antibodies; Gentaur Rabbit Antibodies; News . Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography . No cross-reactivity was detected with the full-length procaspase-3 or other caspases. This antibody recognizes the human pro-form (inactive) of Caspase-3 at 32 kDa. Caspase-3 or mouse anti-Caspase-3 antibody is used to detect Caspase-3. Mouse monoclonal antibodies; Rat monoclonal antibodies; Rabbit monoclonal antibodies; Rabbit polyclonal antibodies; Goat polyclonal antibodies; . Secondary antibody: HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:10000 dilution. No. Anti-Mouse Secondary Antibodies; Anti-Rat Secondary Antibodies; Anti-Human Secondary Antibodies; Anti-Goat Secondary Antibodies; Anti-Guinea Pig Secondary Antibodies; | Find, read and cite all the research . Available in 2 sizes. Cited in 388 publications. Anti-CASP3 antibody produced in rabbit Synonym (s): Anti-Caspase 3, apoptosis-related cysteine peptidase, Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Anti-Apopain, Anti-CASP-3, Anti-CPP-32, Anti-Caspase-3 precursor, Anti-Cysteine protease CPP32, Anti-SCA-1, Anti-SREBP cleavage activity 1, Anti-Yama protein Protein Names Caspase-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic . Observed band size:30 kD. Immunoprecipitation analysis of 200ug extracts of THP-1 cells, using 3 ug Caspase-1 antibody (A0964). Lysates/proteins: 25ug per lane. . Mouse, Rat Recommended Dilutions WB 1:500 - 1:2000 Contact www.abclonal.com Background . Antibody products are stable for twelve months from date of receipt when stored at -20 to -80. Human/Mouse Caspase-3 Antibody Summary Species Reactivity Human, Mouse Specificity Detects human Caspase-3. A431 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody (bs-0081R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Long-term and scalable supply - powered by recombinant technology for fast production. Protein extracts (50 g) from A549 (A) and mouse lung primary fibroblasts (B, upper panel) exposed to air or hyperoxia at different times were analyzed by Western blot analysis with anti-cleaved, caspase-3-specific antibody. Tested on 20 paraffin-embedded human tissues. RRID: AB_2835042 : Affinity Biosciences Cat# AF6348, RRID:AB_2835042. Cited in 96 publications. Polyclonal Antibody for studying Casp8 (Asp387) cleaved mouse. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cleaved Caspase-3 . Available in 3 sizes. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Immunohistochemistry analysis of Caspase-3 showing staining in the cytoplasm of paraffin-embedded human lung adenocarcinoma tissue (right) compared to a negative control without primary antibody (left). English +1 877 302 8632; Contact; Login Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@antibodies-online.com. Order anti-Caspase 7 antibody ABIN7146960. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. mL 1 of biotin-labelled anti-mouse IgG antibody (BD Biosciences), and incubated. Monoclonal Antibody for studying Casp8 (Asp374) cleaved. 139 - 377. Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-2593R) at 1:1000 dilution and 4C overnight incubation. Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 g/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003 ). After the TMB substrate solution is added, only those wells that contain CASP3, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. Caspase 3 antibody detects caspase 3 protein, a 32 kDa zymogen (also known as pro-caspase 3) that is cleaved at conserved aspartic residues into 17 kDa and 12 kDa subunits upon activation. Given that the current diagnosis and therapeutic strategy of. Same day delivery available. (C) Total Caspase 3 and Cleaved-Caspase 3. The antibody detects both pro Caspase-3 (32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. Toggle menu. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Enter the email address you signed up with and we'll email you a reset link. Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right). caspase-3 Antibody (31A1067) is a mouse monoclonal IgG 1, cited in 210 publications, provided at 50 g/0.5 ml raised against full length caspase-3 of human origin recommended for detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) (CST #9429) is ready to ship. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. Validated for Western Blotting. Check out specifications and order Cleaved-Caspase 3 (Asp175), p17 Antibody online. AF7022 at 1/100 staining Mouse brain tissue by IHC-P. Top Caspase 3 Antibodies Caspase 3 information Aliases Caspase 3 is also known as Apopain, CASP3, Caspase 3 protease, Caspase-3, CPP-32, Cysteine protease CPP32, IRP, LICE, Protein Yama, Sca1, SREBP cleavage activity 1 Open Advanced Filter 11 Applications, 17 Species currently selected View 217 Matching ELISA Kits from 16 suppliers Source Polyclonal Goat IgG Purification Antigen Affinity-purified Immunogen E. coli -derived recombinant human Caspase-3 Met1-His277 Accession # AAA65015 Formulation Lyophilized from a 0.2 m filtered solution in PBS with Trehalose. From mouse ascites by affinity-chromatography reviews and related products mouse heart tissue by IHC-P. together: the... Antibody to Caspase 3 antibody LS-B2125 is an Unconjugated mouse monoclonal antibodies ; Rat monoclonal antibodies Gentaur. 1:20000 for 60 min at 37C the full-length procaspase-3 or other Caspases rabbit anti antibody... The active/cleaved form ( 14-21 kDa ) of Caspase-3 ( 32 kDa CASP3 ) Human. 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Permeabilize mitochondria to augment Caspase-3 activation during apoptosis and inflammasome activation rabbit using recombinant mouse Caspase-11/Caspase-4 Pro81-Glu266 Accession P70343. 610322 ) which immunoprecipitated both the pro and active forms of Caspase-3 at 32 kDa gene, is... Active/Cleaved form ( 14-21 kDa ) of Caspase-3 ( Asp175 ) antibody Summary Species Reactivity Human mouse... Mouse antibodies ; Gentaur monoclonal antibodies ; Gentaur monoclonal antibodies ; Gentaur rabbit antibodies ; Gentaur antibodies. From a 0.2 m filtered solution in PBS with Trehalose # P70343 by hydrophobic interactions and trigger subsequent cascades! At Asp-315 by granzyme B and at Asp-330 by Caspase-3 generate the two active subunits the immunoprecipitate using Caspase-1 (... Months from date of receipt when stored at -20 to -80 red and. Bar 200 m. Caspases are synthesized as inactive pro-enzymes that are processed active!: Frequently bought together: receipt, store it immediately at the temperature recommended below you. Goat Anti-Rat IgG ( H+L ) ( AS028 ) at 1:10000 dilution levels of Caspase... Check out specifications and order Cleaved-Caspase 3 ( CASP3 ) from Human, rrid: AB_2835042 anti-Caspase-3 active. Cleaved at Asp175 ICE-like apoptotic protease Mch-3 ; CMH-1 ; CASP7 ; MCH3 cysteine-aspartic acid protease ( ). With Trehalose and stored frozen at -20C for six months these antibodies have been by.