Raman spectroscopy with a range of excitations (532, 633, 785 and 1064 m) and an emphasis on microscopy for small or heterogeneous samples. Sample preparation for surface area/porosity analysis. SCIEX TripleTOF 6600 LC-MS/MS Mass Spectrometer. Qubit Q33226) Wi-Fi Dongle (Cat. swollen polymers, swollen beads, tissue samples). The Helixyte Green All reagent (Ex/Em = 509/529 nm) is a sensitive fluorescent nucleic acid probe for measuring the total quantity of nucleic acids in a sample that may contain dsDNA, ssDNA, RNA, and long oligonucleotides. Inverted materials microscope for metallography in brightfield and polarised light including scanning stage and manual focus drive. UV-Vis Spectrophotometers However, if the assay tube remains in the Qubit WebRNA reagent and the Qubit RNA buffer at room temperature and insert all assay tubes into the Qubit 2.0 Fluorometer only for as much time as it takes for the instrument to measure the fluorescence, because the Qubit 2.0 Fluorometer can raise the temperature of the assay solution significantly, even over a period of a few minutes. Bismuth analytical sources and argon gas cluster ion source for depth profiling of organic and other delicate samples. Connecting a Qubit 3.0 or 4 Fluorometer to a computer running the Windows operating system requires installation of a driver. Central Analytical Research Facility Max 470 nm Blue LED, Max 635 nm Red LED, DNA Quantification, RNA Quantification, Protein Quantification, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, Fluorometers and Fluorescence Polarization Systems. E.A. Q32856) for the Qubit 4 Fluorometer and 8 x 200 L tube strips (Cat. Our webinar series with experienced alumni sharing real world career advice. 6. Unique mixing performance with excellent temperature control to guarantee complete, dependable, and reproducible assay results. High speed confocal imaging. UMI is an acronym for Unique Molecular Identifier. Pricing above applies to assisted use only. Powder diffractometer with parallel beam/thin film capability. The instrument searches the USB drive for assay file(s) and displays their names on the Import new assay screen. Connect the Qubit Fluorometer to your computer using a USB cable. 36-position sample changer; Mirrors for Divergent, Parallel, Focusing beams; 0D/1D/2D Pixel Area Detector; Capillary/foil Transmission; X-ray Reflectometry. We have access to a wide range of sample preparation equipment, including fusion apparatus, and crushing, grinding and micronising equipment. Various analytical balances with precision to 0.1 mg and 0.01 mg, as well as an anti-static kit. You can use the MyQubit Assay Design Tool to create the MyQubit .qbt file that defines your new assay. Take your development further with advanced learning and award pathways. Our state-of-the-art equipment allows for: Our nuclear magnetic resonance and electron paramagnetic resonance spectrometers allow us to investigate the structure, dynamics and interactions of materials at the molecular scale. Analysis of all elements from sodium to uranium, in a wide range of samples, such as soils, sewage sludge, additives in oil, cement, slag, and electronic components and parts. Your goal is to adjust N and K to achieve the lowest possible Quality score as shown in the example. Qubit The Qubit 4 Fluorometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. Thermo Fisher Scientific There are two main methods used to measure molecular vibrations: Materials with strong IR spectra tend to give weak Raman spectra and vice versa. Create Account. Learn about QUTs involvement in collaborative research initiatives. Simbolo M, Gottardi M, Corbo V, et al. The Qubit 2.0 Fluorometer Chemisorption using hydrogen and oxygen also available. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 L and 20 L is acceptable), and read the concentration using a Qubit Fluorometer. Excellent for desktop analysis presentations and publications. I already have a Thermo Scientific NanoDrop instrument. Qubit Youll see your choice on the Verification screen (step 8). Simultaneous disruption of up to 48 biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide or glass beads. Read about our goals for future growth, achievements and success, and the organisational structures that support us. Our laboratory houses cutting-edge mass spectrometry instrumentation interfaced with liquid chromatography and gas chromatography capabilities. Simply add your sample (any volume between 1 L and 20 L is acceptable) and read the concentration using a qubit 2.0, 3, or 4 fluorometer. Technical Resources for Qubit Fluorometers No. Why should I use a Qubit Fluorometer? The instrument will display the Manual, customisable production of tissue microarray blocks, which can consolidate 10 to 100 target samples into a single paraffin block. TESCAN Mira3 Scanning Electron Microscope. Q33252) for the Qubit Flex Fluorometer no. Acknowledging exceptional contributions from our student leaders. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. For the Qubit Flex Fluorometer, use the Qubit Flex Assay tube strips,which come as 8 x 200-L thin-walled polypropylene tubes with very low fluorescence. We provide next-generation proteomics underpinned by data-independent acquisition (DIA) mass spectrometry and advanced computational approaches. Surface area (BET), pore size distribution (<800 angstroms) of solid materials using nitrogen or argon. Qubit Fluorometers detect fluorescent dyes specifically bound to the target molecule. Our equipment and services are available to QUT staff and students, external researchers and commercial clients. WebPopular. As you become familiar with the assays, the instrument, your pipetting accuracy, and significant temperature fluctuations within your laboratory, you can determine your level of comfort with using the calibration data stored from the last time the instrument was calibrated. Training is provided for staff and students to independently conduct their cell analysis research. Near-, Mid- and Far-IR capability. Low resolution XY view stage. To create custom MyQubit assays for the Qubit 2.0 Fluorometer, see Resources for previous Qubit models. Perkin Elmer Optima 8300 DV Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). Designed for bioscience researchers with a wide variety of needs, this microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. Prices include labour unless stated otherwise. The idea seems to have been first implemented in an iCLIP protocol (Knig et al. I already have an older Qubit Fluorometer. Thermo Fisher Scientific Our equipment and services are available to QUT staff and students, external researchers and commercial clients with both self-service (unassisted) and full-service (with technical assistance) options. UV-Vis Spectrophotometers NanoDrop instruments use UV absorbance to measure DNA and RNA concentrations. Thermo Scientific NanoDrop One c Spectrophotometer with WiFi and Invitrogen Qubit 4 Fluorometer. Qubit reagents bind selectively to DNA, RNA, or protein and only emit a fluorescent signal when bound to the target. Independent operation by external users is unavailable. This Fluorometer is the next generation of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. Cutting undamaged 3mm disk samples for transmission electron microscopy. A compact, benchtop spectrophotometer for plate-based high-speed quantification of DNA, RNA or protein samples. 1.5 mm and 2.4 mm sieves for preparation of samples for Mastersizer. Fitted with a 2 micron radius diamond stylus and a 50mm ceramic vacuum chuck. Electrochemical characterisation, surface profiling, work function. Qubit Latest-generation ToF-SIMS instrument for high-sensitivity elemental and molecular mapping of sample surfaces. Webreagents exhibit high photostability in the Qubit Fluorometer, showing <0.3% drop in fluorescence after 9 readings and <2.5% drop in fluorescence after 40 readings. The assay detects oligonucleotides or long ssDNA, and common contaminants such as salts, solvents, detergents, and protein are well tolerated. Hamilton, Hubbard use INBRE grant to bring new instruments to Twelve analyzing crystals are distributed among five wavelength dispersive spectrometers (WDS) including a large crystal "L" type and two high count rate "H" types. When not logged into a user profile, these menu options will appear to be inactive. We invite you to contact Technical Support if you have additional questions or need more information. For each assay, you have the choice to run a new calibration or to use the values from the previous calibration. High precision piezo XYZ mapping stage 200x200x20 m. The Reagent Calculator helps determine how much reagent and buffer to use in your working solution. We can provide support for all aspects of the genomics sample preparation and analysis workflow, including DNA/RNA extraction, library preparation, quality control, manual and automated sample processing, Next-Generation-Sequencing, Microarrays and bioinformatics analysis. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes. Milestone Ultrawave Single Reaction Chamber (SRC). With optimized Qubit Assays, they can distinguish dsDNA from ssDNA or intact from degraded RNA, even in extremely small amounts or in the presence of contaminants. PANalytical AXIOS Wavelength X-ray Fluorescence (WD-XRF) Spectrometer. Fitted with a 60 mm Thermo Ultra Dry EDS Detector. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes. USER GUIDE Chromium Next GEM Automated Single Cell Micromeritics ASAP 2020 Surface Area and Porosity Analyzer. Check out these free resources for background and support materials about Qubit Fluorometric DNA and RNA quantification. Measures film thickness in single- and multi-layer systems, as well as refractive index and dielectric properties. Qubit However, if the assay tube remains in the Qubit TRIzol Reagents; Antibodies; ELISA Kits; Most Popular Categories. to samples by authorised stakeholders in local and remote locations. Lipofectamine Reagents; Instrumental de laboratorio Nalgene; Oligonucleticos, primers, sondas y nucletidos; Pipettes & Pipette Tips; Transcriptasa reversa SuperScript; Ensayos de PCR en tiempo real TaqMan; TRIzol Reagents; Anticuerpos; Kits de ELISA; Categoras ms populares. analysis packages for commercially produced or in-house generated tissue micro-arrays. The Qubit Protein BR Assay is compatible with samples that contain up to 5% detergents. Automated feature detection and analysis software. TESCAN Tima Field Emission Scanning Electron Microscope. Q32856) for the Qubit 4 Fluorometer and 8 x 200-L tube strips ContactChristina Theodoropoulos to discuss how the Cell Analysis Facility can assist in your research, and to make a booking. Multiplexed, single-molecule, epigenetic analysis of plasma Qubit Gatan Model 691 Precision Ion Polishing System (PIPS). Our microscopy laboratory has a suite of routine and advanced light microscopes and electron microscopes that enable users to conduct innovative research. UMIs are complex indices added to sequencing libraries before any PCR amplification steps, enabling the accurate bioinformatic identification of PCR duplicates. Continuum IR microscope with computerised X,Y,Z-stage. Matsuzawa MXT70 Micro Vickers Hardness Tester. Simply dilute the reagent using the buffer provided, add your sample, and read the concentration using a Qubit or other fluorometer. The Central Analytical Research Facility (CARF) is located across QUT campuses, with the main node situated in the Science and Engineering Centre at our Gardens Point campus. A minimum of 10 slides is required for all paraffin sectioning and staining requests. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent. Ultra-high-resolution pole piece, Gatan Orius SC1000 CCD camera, LaB6 gun. The Varioskan LUX multimode reader has received
Raman spectroscopy which involves the inelastic scattering of higher energy photons. Infrared spectroscopy (IR or FT-IR) which involves the absorption of an infra-red photon. With optimized Qubit Assays, they can distinguish dsDNA from ssDNA or intact from degraded RNA, even in extremely small amounts or in the presence of contaminants. Accurate readings Since the Qubits reagents are specific to their target, the presence of contaminants in samples will not interfere with the concentrations. Depending on sample volume, the assay kit is designed to be accurate for initial RNA sample concentrations of 0.2 to 200 ng/L, providing a detection range of 4200 ng. Multiplexed, single-molecule, epigenetic analysis of plasma These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides. Simply enter the fluorescence values obtained in Fluorometer mode on the Qubit Fluorometer, along with the corresponding concentrations of the analyte. Sample preparation for surface area and porosity analysis through gas absorption/desorption, with vacuum mode that prepares samples by heating and evacuation. Q33252) for the Qubit Flex Fluorometer Lu R, Zhao X, Li J. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Qubit Assays WebDNAzol Reagent, for isolation of genomic DNA from solid and. The system features a monochromated Al K X-ray source and a helium lamp for UPS measurements on conduction bands. Qubit dsDNA BR Assay Kit Notes Qubit dsDNA HS and BR Assay kits can be used with any Qubit Fluorometer Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. No. Extensive searchable polymer library for polymer ID (>5700 spectra), common materials and forensics (>6500 spectra) and small molecules (>9000 spectra). 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